Investigation of Respiratory Control in Dormant Grapevine Bud

Grapevine is a temperate perennial, with a reproductive cycle spanning two seasons (ca. 18 months). Normal, or optimal transitions from dormancy to bud burst, flowering, fruit ripening, senescence and so on, act in synchrony with seasonal changes in climate. The optimal regulation of bud dormancy is poorly understood by comparison to flowering or fruit ripening, despite bearing the floral organs that determine yield potential. Current knowledge is limited to either agronomic data, at one end of the scale, or gene expression data at the other. This project sought to bridge this gap by translating knowledge and technology from the more fundamental plant sciences, to the more complex grapevine bud. There was a need for basic understanding of how primary metabolism is regulated by temperature, and the work this project supported provides a firm platform. This project has enabled techniques in whole organ respiration, and the oxidative state of bud tissue to be developed and applied during bud burst.